Concentration (μg/mL) Mean Absorption (n = 5) Concentration Recovered (μg/mL) Mean % Accuracy 200 0.804488 199.747 99.8735 100 0.404977 99.8693 99.8693 50 0.207938 50.6095 101.219 25 0.104988 24.872 99.488 12.5 0.053894 12.0985 96.788

Table 3: Analytical method validation parameters for accuracy.