Time (min) |
Mobile phase A (%) |
Mobile phase B (%) |
Comment |
0.0 |
80.0 |
20.0 |
Isocratic hold to elute positively charged peptide and hydrophilic formulation excipients |
4.0 |
80.0 |
20.0 |
4.1 |
0.0 |
100.0 |
Gradient step to elute heterogeneous and lipophilic polysorbates in one single peak |
9.0 |
0.0 |
100.0 |
9.1 |
80.0 |
20.0 |
Column re-equilibration |
12.0 |
80.0 |
20.0 |
|