Step Mixture (68:32) Mixture (70:30) Mixture (74:24) Mixture (76:24) H2O (tot) 1 100       32.00 2 81.38 18.62     31.63 3 52.59 47.41     31.05 4 33.98 66.02     30.68 5 21.96 78.04     30.44 6 14.19 67.19 18.62   29.54 7 9.17 43.42 47.41   28.29 8 5.92 28.06 66.02   27.48 9 3.83 18.13 78.04   26.96 10 2.47 11.71 85.81   26.61 11 1.6 7.57 72.21 18.62 26.03 12 1.03 4.89 46.66 47.41 25.31 13 0.67 3.16 30.15 66.02 24.85 14 0.43 2.04 19.48 78.04 24.54 15 0.28 1.32 12.59 85.81 24.35

Table 1: Linear gradient for 15 steps based on mixture of acetonitrile with water for glucose, lactose and GOS separation.