Pig Dilution Endotoxemia* Sample % Sample % Sample % Mean ± SEM (EU/mL) Variation coefficient >25% Spike recovery<50% Spike recovery>200 % n=21 1/10 1.2± 0.4 48 67 0 n=21 1/40 28.6± 11.6 14 24 67 n=63 1/100 16.0± 6.3 27 22 14 n=211 1/200 16.7± 3.6 15 10 17

Table 2: Endotoxemia (EU/mL) measured in pigs at different dilutions and proportion of samples (%) with variation coefficient >25%, recovery <50% and >200%.