Primary sequence

Peptide	*Precursor ion (m/z)*	*Product ion (m/z)*
HC-3-1024 mAb	694.4 [M+3H]³⁺	916.5 y₉⁺¹ ion
HC-3-1024 mAb	694.4 [M+3H]³⁺	866.5 b₈⁺¹ ion
HC-1-992 mAb	674.7 [M+3H]³⁺	648.8 y₁₃⁺² ion
HC-1-992 mAb	674.7 [M+3H]³⁺	712.9 y₁₄⁺² ion
HC-1-992 mAb	674.7 [M+3H]³⁺	777.0 y₁₅⁺² ion
HC-3-total	603.3 [M+3H]³⁺	805.4 y₁₄⁺² ion
HC-3-1024 mAb IS	696.7 [M+3H]³⁺	859.4 y₈⁺² ion
HC-1-992 mAb IS	677.3 [M+3H]³⁺	714.4 y₁₄⁺² ion
HC-3-total IS	605.3[M+3H]³⁺	807.4 y₁₄⁺² ion

Table 2: MRM transitions and charge states for surrogate peptides and the stable isotopes (internal standards).