mAb Sequence #of aa MW Da pI "Macaca fascicularis BLASTP” "Homo sapiens BLASTP" Identity% vs. HC of the other mAb 992 EVQLQQPGSELVRPGASVK 19 2022.2 6.24 OK OK 78.9 992 ASGYTFTSYWMHWVK 15 1864.1 8.55 OK "Not OK” Chain B, Mature Metal Chelatase Catalytic Antibody With Hapten pdb|3FCT|B 93.3 992 QRPGQGLEWIGNIYPGSR 18 2028.2 8.75 OK OK 82.4 992 ATLTVDTSSSTAYMQLSSLTSEDSAVYYCTR 31 3352.6 4.03 OK OK 90.3 992 NGDYYVSSGDAMDYWGQGTSVTVSSASTK 29 3034.1 3.93 OK OK 80.0 1024 QVQLQQPGAELVEPGGSVK 19 1964.2 4.53 OK "Not OK” Chain B, Anti-blood Group A Fv pdb|1JV5|B" 78.9 1024 ASGYTFTSHWMHWVK 15 1838.0 8.65 OK OK 93.3 1024 QRPGQGLEWIGEINPSSGR 19 2081.2 6.14 OK OK 82.4 1024 SSSTAYMQFSSLTSEDSAVYYCVR 24 2682.9 4.37 OK OK 91.7 1024 YYGYDEAMDYWGQGTSVTVSSASTK 25 2766.9 4.03 OK OK 80.0

Table 4: Result summary of the in-silico selection process for Fab Heavy Chains (HC). “OK” and “Not OK” mean “no match” or “match”, respectively, for known protein sequences present in monkey or human proteome. When a match is present, some examples of proteins containing that exact sequence are listed. The level of identity with the corresponding peptide in the same region on the other mAb is reported (I to L not considered) as Identity% score. The average MW, and calculated isoelectric point (pI) are also reported for each peptide.