mAb Sequence #of aa MW Da pI "Macaca fascicularis BLASTP" "Homo sapiens BLASTP" Identity % vs. LC of the other mAb 992 DIQMTQTTSSLSASLGDR 18 1911.0 4.21 OK "Not OK Chain A, Anti-Blood Group A Fv, pdb|1JV5|A, pdb|1IKF|L, pdb|3U0W|L" 50.0 992 TSQDIGNYLNWYQQKPDGTVK 21 2455.6 5.63 OK OK NA 992 LLIYYTSR 8 1028.2 8.59 OK "Not OK”, immunoglobulin VL region=humanized bispecific antibody [human, Peptide Recombinant, 107 aa, gb|AAB24132.1|" NA 992 LHSGVPSR 8 851.9 9.76 Not OK Not OK NA 1024 DIVMTQAAFSNPVTLGTSASISCR 24 2469.8 5.83 OK "Not OK, anti-GlcNAc antibody variable region:SUBUNIT=light chain prf||1911357B" 50.0 1024 FSSSGSGTDFTLR 13 1361.4 5.84 OK OK NA 1024 VEAEDVGVYYCAQNLELPYTFGGGTK 26 2824.1 4.00 OK OK NA

Table 5: Result summary of the in-silico selection process for Fab Light Chains (LC). “OK” and “Not OK” mean no match or match, respectively, for known protein sequences present in monkey or human proteome. When a match is present, some examples of proteins containing that exact sequence are listed. The level of identity with the corresponding peptide in the same region on the other mAb is reported (I to L not considered) as Identity% score. The average MW, and calculated isoelectric point (pI) are also reported for each peptide.