| Peptide Sequence |
Peptide Name |
Program D |
Program DS |
PA: SAPE |
| Round 2 |
Round 3 |
Round 4 |
Round 2 |
Round 3 |
Round 4 |
| WFCFTCPSSSDVIKG |
DS-A09 |
0 |
0 |
0 |
0 |
1 |
2 |
13.2 |
| YTDFVCFTCTMPQLQ |
DS-A14 |
0 |
0 |
0 |
0 |
1 |
2 |
14.8 |
| WSCFTCDHGAETLVS |
DS-A47 |
0 |
0 |
0 |
0 |
1 |
2 |
13.9 |
| TWFCFTCYKAPVKHD |
DS-B30 |
0 |
0 |
0 |
0 |
9 |
10 |
6.5 |
| SYWSCFTCTTLSGFS |
D/DS-A28 |
0 |
4 |
13 |
0 |
3 |
4 |
8.9 |
| FTNWSCFTCSSSTNA |
D/DS-B43 |
0 |
3 |
1 |
0 |
1 |
3 |
8.9 |
| SNWICFTCAFPRETA |
D/DS-E32 |
0 |
9 |
5 |
0 |
2 |
0 |
9.0 |
| PGISEVQWSCFTCIV |
D-E04 |
0 |
12 |
12 |
0 |
0 |
0 |
13.8 |
| VVWIPWTVWTVAPET |
D-E06 |
0 |
2 |
2 |
0 |
0 |
0 |
10.5 |
| STLFYCFTCLSSVGS |
D-E10 |
2 |
13 |
23 |
0 |
0 |
0 |
7.9 |
| SSWLCFTCLQAPAIS |
D-E11 |
3 |
6 |
5 |
0 |
0 |
0 |
8.1 |
| YWHCWTCNSVNTDSR |
D-J06 |
0 |
5 |
2 |
0 |
0 |
0 |
5.4 |
| PFSYLGTLYIPWESF |
D-J40 |
0 |
1 |
2 |
0 |
0 |
0 |
7.6 |
| E. coli cells displaying peptides on an eCPX scaffold were sorted using the autoMACS® separation programs shown and 144 colonies were sequenced for sorting rounds 2 through 4. The number of sequenced colonies expressing each top PA binding peptide candidate is shown for each round. Consensus sequence WXCFTC is shown in bold. PA:SAPE is the ratio of the average nMFI for each, as determined by FACS from 3 independent experiments. |
|
