Tested mobile phase composition composed
H2O (%) 80 90 95 100
Acetonitrile (%) 20 10 5 0
TFA (%) / Sodium perchlorate (mg mL-1) 0/0 0/0 0/0 0/0
0.05/6 0.05/6 0.05/6 0.05/6
0.1/12 0.1/12 0.1/12 0.1/12
0.15/18 0.15/18 0.15/18 0.15/18
Table 1: Tested mobile phase composition composed of water, acetonitrile, trifluoroacetic acid and sodium perchlorate.