Lysis procedure
GSH [µM]
MPER lysis buffer, room temperature
2.8
5% TFA, -18°C
1.7
5% MPA, -18°C
0.9
0.5% picric acid, -18°C
10.3
Table 3:
Amount of extracted GSH by each of the lysis procedures. RSD values were below 15% (N=3).