Minutes Event Parameters
0-0.6 B% 70 (isocratic)
0.6-1.6 B% 70-90 (liner)
0-3.5 Total flow 0.25 ml/min
3.5-3.7 Total flow 0.25-0.6 ml/min
1.6-5.5 B% 90 (isocratic)
5.5-5.6 B% 90-70 (liner)
3.5-5.9 Total flow 0.6 ml/min
5.9-6.0 Total flow 0.25-0.6 ml/min
5.6-7.5 B% 70 (liner)
Table 1: HPLC gradient program.