Chromatogram
Figure 2: Chromatogram showing the SRM of A) Standard adenine (136>>92) showing the peak at 2.9 min, B) Standard adenosine does not show the adenine (136>>92) peak at 2.9 min indicating there is no co-elution. C) Same as B, standard adenine does not shows the adenosine peak (268>>136) at 2.4 min indicating there is no co-elution with adenosine. D) Same as A, standard Adenosine (268>>136) showing the peak at 2.4 min. Indicating nucleobases are unique SRM and not eluting with nucleosides.