Figure 1: DIC imaging of Caulobacter Crescentus. 3D acquisition (A) and corresponding volume reconstruction directly (B) or after processing with the STA (C). While direct 3D reconstruction results in bacteria deformation and artifact (examples highlighted by yellow stars) crucial morphological parameter, like size, shape and curvature are easily retrieved after STA, whatever the original orientation of the bacteria. Stack size: 1004 × 1002 × 52 voxels, voxel size 115 × 115 × 390 nm.