| Factors |
Levels of the investigation |
| (-) |
(+) |
| X1 |
Acetonitrile (%) |
9.1 |
14.5 |
| X2 |
β–CD (mM) |
5.81 |
8.81 |
| X3 |
Flow rate (mlmin-1) |
1 |
1.5 |
| X4 |
Concentration of metoprolol tartarate (mlL-1) |
0.25 |
0.50 |
|
| (-) and (+) are “low” and ”high” levels |
| Table 1: Factor and their levels. |
