Parameters FOS DEX OND GRA r 0.9907 0.9984 0.9991 0.9999 Accuracy (%) 95.1-103.0 101.0-103.1 101.7-102.7 99.3-103.5 Repeatability (%) ≤ 1.9 ≤ 6.0 ≤ 3.2 ≤ 4.1 Intra-day precision (%) ≤ 1.9 ≤ 7.0 ≤ 2.9 ≤ 3.1 Inter-day precision (%) ≤ 4.0 ≤ 6.0 ≤ 5.6 ≤ 6.2 LOD (mg/ml) 0.097 0.011 0.009 0.002 LOQ (mg/ml) 0.323 0.040 0.030 0.006

FOS:Fosaprepitant DEX: Dexamethasone GRA:Granisetron OND:Ondansetron r: correlation coefficient LOD: Limit of Detection LOQ: Limit of Quantification

Table 3: Validation parameters of chromatographic method.