Parameter Live (PI negative) Dead (PI positive) P value ASDE 0.15±0.04 0.11±0.01 <10-3 AA 0.25±0.22 0.03±0.03 <10-3 LFAP 3.25±1.79 1.61±0.19 <10-3 HFAP 1.52±0.07 1.42±0.06 <10-3 LFAP/HFAP 2.12±0.10 1.14±1.11 <10-3 EEE 78.0±4.74 71.82±2.90 <10-3

Table 1: Average GLIE fluctuation arameters: ASDE, AA, LFAP, HFAP, ratio of LFAP to HFAP and EEE in the 10 gradually dying cells before death according to PI flourescence, compared to average GLIE fluctuation parameter values after PI determination of death.