Time (min)
Flow Rate (mL/min)
% Solvent A
% Solvent B
0
0.5
95
5
0.5
0.5
90
10
2.5
0.5
25
75
2.8
0.5
0
100
3.8
0.5
0
100
4.5
0.5
95
5
5.5
0.5
95
5
Table 2:
Gradient used for morphine and ropivacaïne LC analysis.