Time (min) Flow Rate (mL/min) % Solvent A % Solvent B
0 0.5 95 5
0.5 0.5 90 10
2.5 0.5 25 75
2.8 0.5 0 100
3.8 0.5 0 100
4.5 0.5 95 5
5.5 0.5 95 5
Table 2: Gradient used for morphine and ropivacaïne LC analysis.