Time (min)
Flow Rate (mL/min)
% Solvent A
% Solvent B
0
0.5
95
5
0.5
0.5
90
10
0.9
0.5
75
25
2.0
0.5
75
25
2.5
0.5
0
100
3.5
0.5
0
100
3.8
0.5
95
5
5.5
0.5
95
5
Table 3:
Gradient used for ziconotide LC analysis.
