Nominal concentrations Calculated concentrations Between-run accuracy and precision
Run 1 Run 2 Run 3 mean RE% RSD%
Morphine calibrators (mg/mL)
0.10 0.090 0.101 0.099 0.097 -3.2% 6.0%
0.50 0.519 0.502 0.499 0.507 1.3% 2.1%
1.00 1.041 1.001 1.000 1.014 1.4% 2.3%
4.00 4.091 3.924 4.016 4.010 0.3% 2.1%
16.00 16.051 16.073 16.174 16.100 0.6% 0.4%
32.00 31.808 31.998 31.811 31.872 -0.4% 0.3%
R2 0.9999 0.9999 0.9999 0.9999   0.001%
Slope 0.9939 1.0010 0.9961 0.9970   0.361%
Ropivacaïne  calibrators (mg/mL)
0.312 0.285 0.302 0.306 0.298 -4.6% 3.8%
0.625 0.606 0.619 0.619 0.615 -1.6% 1.2%
1.25 1.245 1.245 1.244 1.244 -0.4% 0.1%
2.50 2.529 2.510 2.508 2.516 0.6% 0.5%
5.00 5.054 5.023 5.023 5.033 0.7% 0.4%
10.00 9.969 9.987 9.988 9.981 -0.2% 0.1%
R2 0.9999 0.9999 0.9999 0.9999   0.002%
Slope 1.0000 1.0000 1.0000 1.0000   0.0%
Ziconotide   calibrators (μg/mL)
0.10 0.108 0.110 0.102 0.107 6.8% 3.7%
0.20 0.206 0.217 0.211 0.211 5.6% 2.6%
0.40 0.375 0.362 0.364 0.367 -8.3% 2.0%
1.00 0.919 0.943 0.981 0.948 -5.2% 3.3%
2.00 2.027 1.810 2.087 1.975 -1.3% 7.4%
4.00 4.064 4.258 3.955 4.092 2.3% 3.8%
R2 0.9997 0.9969 0.9995 0.9987   0.2%
Slope 1.0195 1.0517 0.9968 1.0227   2.7%
Table 4: Calibrators between-run accuracy and precision.