| Liquid-liquid extractiona |
Protein precipitation |
| Extraction condition |
PE(%) |
% Ion Suppression |
Precipitating agent |
% Ion Suppression |
| 500 μL of water |
48.9 |
32.2 |
Acetonitrile |
62.6 |
| 500 μL of 1% formic acid in water (v/v) |
67.2 |
18.6 |
1% formic acid in acetonitrile (v/v) |
56.4 |
| 500 μL of 1% Liq. Ammonia in water (v/v) |
57.6 |
24.1 |
1% Liq. Ammonia in acetonitrile (v/v) |
71.3 |
| 750 μL of 1% formic acid in water (v/v) |
74.7 |
12.1 |
Methanol |
52.7 |
| |
1% formic acid in methanol (v/v) |
76.1 |
| 1% Liq. Ammonia in methanol (v/v) |
76.1 |
| aBy using ethyl acetate based on results from Table 1. |
