| Conditioning Optimization |
Washing Optimization |
Elution Optimization |
S/N ratio |
PE (%) |
%Ion suppression |
| 0.5 mL of MeoH followed by 0.5mL of water |
1 mL of MeoH followedby 1mL of water |
1 mL of 10% of Liq.NH3 in MeoH |
Fail |
81.4 |
8.1 |
| 0.5 mL of MeoH followed by 0.5mL of water |
1 mL of MeoH followed by 1 mL of water |
1 mL of 10% of Liq.NH3 in ACN |
Fail |
89.5 |
4.9 |
| 0.5 mL of MeoH followed by 0.5mL of water |
1 mL of 1% formic acid in water (v/v) followed by 1 mL of MeoH |
1 mL of 10% of Liq.NH3 in ACN |
Fail |
95.5 |
3.6 |
| 1 mL of 10% of Liq.NH3 in ACN and 1 mL of 1% Formic acid in water |
1 mL of 1% formic acid in water (v/v) followed by 1 mL of MeoH |
1 mL of 10% of Liq.NH3 in ACN |
Fail |
99.5 |
0.5 |
| Preparation of sample:750 μL of 1% formic acid in water solution as pretreatment for 100 μL of plasma based on results from Table 2. |
