Matrix type Ion enhancement/Ion suppression of Niacinin API-3000 ion source across the QC levels
LQC MQC HQC
SPE LLE SPE LLE SPE LLE
NP 2.55 9.20 -0.31 7.35 -0.91 4.26
HP 1.47 12.65 1.99 7.76 0.29 10.59
LP 1.53 11.12 0.81 8.30 -0.97 10.89
Matrix type Ion enhancement/Ion suppression of Niacinin API-3200 ion source across the QC levels
LQC MQC HQC
SPE LLE SPE LLE SPE LLE
NP -19.38 -41.45 -31.45 -65.28 -26.53 -52.94
HP -23.71 -78.92 -31.59 -21.43 -46.42 -67.52
LP -17.78 -72.03 -32.27 -49.74 -34.69 -58.99
Matrix type Ion enhancement/Ion suppression of Niacinin API-4000 ion source across the QC levels
LQC MQC HQC
SPE LLE SPE LLE SPE LLE
NP 4.58 10.97 -0.87 11.81 1.38 12.15
HP 0.91 10.85 0.73 19.02 -0.37 10.85
LP -0.04 12.65 1.78 16.42 0.07 11.61

NP=Normal Plasma; HP=Hemolyzed Plasma; LP=Lipemic Plasma

Positive value indicates ion-suppression and negative value indicate ion-enhancement

Table 4: Ion-enhancement/ion-suppression for niacin across QC level analyzed on different API models prepared with different extraction techniques (n=6).