Time % of Mobile phase-A % of Mobile phase-B Total flow rate (mL/min)
0.00 100.0 0.0 1.8
6.20 100.0 0.0 1.8
6.21 0.0 100.0 1.8
17.00 0.0 100.0 1.8
17.01 100.0 0.0 1.8
18.00 100.0 0.0 1.8
Table 1: Binary flow program.