Time
% of Mobile phase-A
% of Mobile phase-B
Total flow rate (mL/min)
0.00
100.0
0.0
1.8
6.20
100.0
0.0
1.8
6.21
0.0
100.0
1.8
17.00
0.0
100.0
1.8
17.01
100.0
0.0
1.8
18.00
100.0
0.0
1.8
Table 1:
Binary flow program.
