Overview of Method Development Exercises
Analyte Information
• Structure
• Properties
Method Optimization
QC Preparation Appropriate to Range
Evaluation of Extraction Strategies (Protein Precipitation, LLE/SLE, SPE)
• Phospholipid impact evaluation
• Recovery
• Matrix Stability
• Extract Stability
• Initial Carryover Evaluation
Post-column infusion experiment
Mock Validation Run
• Accuracy and Precision
• Carryover
• Freeze / Thaw Stability
• Bench Top (pre-processed) Stability
• Long Term Stability
• Recovery
• Matrix Effect
• Selectivity
Length of run should mimic longest expected sample set, padded with blanks
Table 1: Overview of Method Development Exercises.