Figure 7: Expression of LXR-α, IFNγ and LDLR after exposure of PBMCs derived from CHD patient with various ligands. +22R(OH)=10M, Withaferin A=10M , Vitamin D3=50ng/ml. A) Representative agarose gel photographs showing ethidium bromide stained RT-PCR products. B) The signal intensities of these RT-PCR products were measured using SCION IMAGE analysis software. The relative levels of target mRNA expression were determined by normalizing their individual band intensity to β actin band intensity. Each bar represents mean SD for the combined results of three separate experiments. (Statistical significance is shown by **= p<0.05).