Figure 8: Effect of vitamin D3 (50ng/ml) on LXR-α knock out PBMCs derived from CHD patients. Control is the patient PBMCs transfected with scrambled siRNA sequence. Right panel shows representative agarose gel photographs showing ethidium bromide stained RT-PCR products and the left panel shows signal intensities of these RT-PCR products using SCION IMAGE analysis software. The relative levels of target mRNA expression were determined by normalizing their individual band intensity to β actin band intensity. Each bar represents mean SD for the combined results of three separate experiments. (Statistical significance is shown by **= p<0.05).