S.No. |
Gene and PCR product size |
Primer Pair |
No of PCR Cycles |
1 |
LXR-α
(169bp) |
Forward: 5’- CAGATTGCCCTGCTGAAGAC -3’
Reverse: 5’- GAACTCGAAGATGGGGTTGA -3’ |
38 |
2
|
PPARγ
(474bp) |
Forward: 5’- TCTCTCCGTAATGGAAGACC -3’
Reverse: 5’- GCATTATGAGACATCCCCAC -3’ |
38 |
3 |
CD36
(389bp) |
Forward: 5’- GAGAACTGTTATGGGGCTAT -3’
Reverse: 5’- TTCAACTGGAGAGGCAAAGG -3’ |
26 |
4 |
LDLR
(258bp) |
Forward: 5’- CAATGTCTCACCAAGCTCTG -3’
Reverse: 5’- TCTGTCTCGAGGGGTAGCTG -3’ |
31 |
5 |
CX3CR1
(410bp) |
Forward: 5’- CCGAGGTCCTTCAGGAAATCT -3’
Reverse: 5’- TCAGCATCAGGTTCAGGAACTC -3’ |
27 |
6 |
IL-8
(300bp) |
Forward: 5’-ATGACTTCCAAGCTGGCCGTGGCT -3’
Reverse: 5’- TCTCAGCCCTCTTCAAAAACTTCT -3’ |
30 |
7 |
IFNγ
(423bp) |
Forward: 5’- CGTTTTGGGTTCTCTTGGCTGTT -3’
Reverse: 5’- CTCCTTTTTCGCTTCCCTGTTTT -3’ |
35 |
8 |
β actin
(256 bp) |
Forward: 5’- TCCTTAATGTCACGCACGAT -3’
Reverse: 5’- CATGTAGGTTGCTACCAGGC -3’ |
20 |
