Before furosemide and before  intraperitoneal medication Medication at 15 min before furosemide   Time after furosemide  (post-furosemide time) (min) 95  100 110 120 130 140 150   Time after medication application (post-therapy time)  (min)   ECG amplitudes  of  P, R, S, T waves (0.01mV); P wave duration, QRS, QT, intervals  duration (msec) 110 115 125 135 145 155 165 P wave amplitude (0.01mV) 2.6±0.8 CONTROL (saline 5ml/kg i.p.) 3.4± 0.8 3.3± 1.1 3.4± 0.5 3.4± 0.9 3.5± 1.1 3.4± 1.1 3.4± 1.3 2.7±0.4 BPC 10μg/kg i.p. 2.5± 1.1 2.5± 0.8 2.5± 1.4 2.5± 1.5 2.5± 0.9 2.5± 1.2 2.5± 1.1 2.5±0.3 BPC 10ng/kg i.p.   2.7± 0.6 2.7± 1.1 2.7± 0.5 2.7± 0.2 2.7± 1.1 2.7± 1.2 2.6± 1.1 R wave amplitude (0.01mV)   56±2.1 CONTROL (saline 5ml/kg i.p.) 39.2± 3.2 36.1± 2.7 36.0± 2.1 35.2± 3.2 35.1± 2.7 35.8± 2.2 35.7± 1.7 58.9±1.3 BPC 10μg/kg i.p.   51.0± 2.2 † 51.5± 1.6 † 50.9± 1.3  † 50.1± 2.1 † 50.5± 1.6 † 51.1± 3.1 † 52.5± 1.6 † 56.3±0.2 BPC 10ng/kg i.p.   55.2± 1.1 † 55.3± 1.2 † 54.3± 1.2  † 53.5± 2.1 † 53± 1.2 † 53± 1.9 † 53.2± 1.2 † S  wave amplitude (0.01mV) 21.4±1.8 CONTROL (saline 5ml/kg i.p.) 13.2± 2.4 12.9± 3.6 12± 1.8 12.2± 2.4 12.1± 3.6 12.4± 1.9 12.6± 2.6 21.9±0.6 BPC 10μg/kg i.p.   22.6± 0.8 † 22.4± 1.5 † 22.9± 0.6  † 22.6± 0.8 † 23.4± 1.7 † 23.4± 0.8 † 23.1± 1.4 † 22.3±1.1 BPC 10ng/kg i.p.   21.1± 1.1 † 23± 0.2 † 22.3± 1.1  † 21.1± 1.1 † 23± 0.6 † 22.2± 1.1 † 23.2± 1.6 † T  wave amplitude (0.01mV) 2.3±0.1 CONTROL (saline 5ml/kg i.p.) 1.5± 0.1 1.4± 1.3 1.4± 0.7 1.4± 0.3 1.4± 0.6 1.4± 0.6 1.4± 0.2 2.1±0.3 BPC 10μg/kg i.p.   2.5± 1.1 † 2.5± 0.9 † 2.4± 1.1  † 2.5± 0.9 † 2.6± 1.1 † 2.5± 1.4 † 2.6± 1.3 † 2.2±0.4 BPC 10ng/kg i.p.   2.1± 0.1 † 2.1± 0.9 † 2.1± 0.4  † 2.1± 0.9 † 2± 0.8 † 2.1± 1.1 † 2.2± 0.4 † P  wave duration (msec) 17±0.9 CONTROL (saline 5ml/kg i.p.) 25± 1.2 26.1± 2.2 27.2±1.4 27.8± 1.6 28.3± 1.6 28.5± 2.8 26.9± 1.2 16±1.1 BPC 10μg/kg i.p.   17± 1.1 † 17.4± 0.9 † 17.9± 0.8 † 17.6± 0.7 † 17.9± 1.7 † 18± 2.1 † 18.1± 1.4 † 17±1.3 BPC 10ng/kg i.p.   18± 1.3 † 17.1± 0.6 † 18± 1.4 † 19.1± 1.2 † 19.2± 1.5 † 19.3± 0.9 † 19.3± 1.4 † QRS  interval duration (msec) 16±0.3 CONTROL (saline 5ml/kg i.p.)¥ 30± 1.1 31.8± 1.1 32.2± 1.1 32.7± 1.5 32.9± 2.2 33.2± 1.6 33.7± 1.4 15±1.1 BPC 10μg/kg i.p.¥   17.1± 0.9 † 15.5± 1.7 † 15.5± 1.4 † 14.6± 0.7 † 14.5± 0.9 † 14.3± 1.4 † 14.1± 0.8 † 16±0.8 BPC 10ng/kg i.p. ¥ 20± 1.2 † 19.8± 1.3 † 17.5± 2.4 † 16.4± 0.7 † 16.1± 0.7 † 15.7± 0.9 † 14.9± 0.7 † QT interval duration (msec) 82±1.2 CONTROL (saline 5ml/kg i.p.)¥ 101± 1.1 103± 1.8 104.9± 0.7 105.7± 1.1 105.5± 0.9 105.6± 0.7 105.1± 0.5 88±0.8 BPC 10μg/kg i.p.¥   75± 2.5 † 71.1± 2.4 † 70.8± 1.3 † 70.6± 0.9 † 70.3± 0.8 † 70.1± 1.2 † 70.2± 0.6 † 85±1.5 BPC 10ng/kg i.p.¥   78± 3.1 † 73.1± 1.4 † 72.8± 1.8 † 71.8± 1.4 † 71.2± 0.9 † 71.1± 0.9 † 70.8± 1.4 †

Table 3A: Effects of hypokalemia on amplitudes (0.01mV) of ECG waves, ECG wave duration and interval duration (msec). BPC 157 administered intraperitoneally was given prophylactically before furosemide (100 mg/kg intraperitoneally). Values are mean ± SEM. ¥ - Two-way ANOVAs with repeated measure on one factor (P<0.05) difference between measurements depends on group membership. †P<0.05 significantly different from control values.