90 min after furosemide, before intraperitoneal medication Medication at 90 min after furosemide Time after furosemide (post-furosemide time) (min) 95 100 110 120 130 140 150 Time after medication application (post-therapy time) (min) ECG amplitudes of P, R, S, T waves (0.01mV); P wave duration, QRS, QT intervals duration (msec) 5 10 20 30 40 50 60 P wave amplitude (0.01mV) 3.5±0.3 CONTROL(saline 5ml/kg i.p.) 3.3± 0.3 3.5± 1.2 3.4± 0.9 3.5± 1.1 3.6± 1.3 3.6± 1.2 3.4± 1.3 3.3±0.1 BPC 10μg/kg i.p. 2.6± 0.15 2.7± 0.2 2.7± 0.9 2.8± 1.3 2.9± 0.4 2.9± 0.8 2.5± 1.1 3.3±0.2 BPC 10ng/kg i.p. 2.4± 0.3 2.6± 0.2 2.7± 0.7 2.7± 1.1 2.8± 0.9 2.8± 1.4 2.6± 1.1 R wave amplitude (0.01mV) 33.2±0.7 CONTROL(saline 5ml/kg i.p.) 32.18± 0.5 33.2± 0.7 33.4± 0.3 32.5± 0.5 32.1± 0.7 32.6± 0.8 35.7± 1.7 32.3±0.4 BPC 10μg/kg i.p. 40.9± 0.4 † 41.4± 1.2 † 41.1± 1.1 † 41.6± 1.2 † 41.4± 1.3 † 41.5± 1.5 † 42.5± 1.6 † 32.5±0.6 BPC 10ng/kg i.p. 41.4± 0.5 † 41.7± 0.6 † 41.3± 1.2 † 41.5± 1.4 † 41.4± 1.4 † 41.4± 0.9 † 43.2± 1.2 † S wave amplitude (0.01mV) 14.1±0.6 CONTROL(saline 5ml/kg i.p.) 14.6± 0.9 15.2± 0.9 15.1± 0.7 15.2± 0.3 15.6± 1.1 15.3± 1.2 12.6± 2.6 14.3±0.2 BPC 10μg/kg i.p. 22.2± 0.7 † 20.9± 0.4 † 21.5± 1.1 † 22.7± 1.3 † 23.2± 1.5 † 23.6± 1.7 † 23.1± 1.4 † 13.9±0.5 BPC 10ng/kg i.p. 22.2± 0.5 † 20.9± 0.5 † 21.1± 0.9 † 21.3± 1.1 † 21.4± 0.4 † 21.5± 0.9 † 23.2± 1.6 † T wave amplitude (0.01mV) 1.3± 0.1 CONTROL(saline 5ml/kg i.p.) 1.4± 0.2 1.3± 0.1 1.2± 0.9 1.2± 1.1 1.2± 0.7 1.2± 0.3 1.3± 0.3 1.3± 0.3 BPC 10μg/kg i.p. 2.2± 0.4 † 2.3± 0.4 † 2.3± 0.8 † 2.4± 0.1 † 2.4± 0.6 † 2.4± 0.5 † 2.4± 0.2 † 1.4± 0.1 BPC 10ng/kg i.p. 2.5± 0.1 † 2.4± 0.2 † 2.3± 0.1 † 2.2± 0.2 † 2.4± 0.4 † 2.3± 0.3 † 2.4± 0.1 † P wave duration (msec) 23.5± 0.8 CONTROL(saline 5ml/kg i.p.) 22.6± 0.4 23.5± 0.3 23.4± 0.4 23.9± 0.9 23.6± 1.1 24.3± 0.8 24.7± 1.2 22.7± 0.4 BPC 10μg/kg i.p. 18±0.6 † 18.1±0.1 † 17.8±0.4 † 18.1±0.1 † 17.2± 1.1 † 18.3±0.4 † 18.3± 0.4 † 23.4± 0.4 BPC 10ng/kg i.p. 18.8± 0.3 † 19.1± 0.2 † 19.1± 0.4 † 19.2±0.6 † 19.3± 0.9 † 19.2± 0.7 † 19.1± 1.1 † QRS interval duration (msec) 30.3± 0.6 CONTROL(saline 5ml/kg i.p.) 31.5± 0.8 31.8± 0.4 32.4± 1.1 32.7± 0.9 32.7± 0.2 32.5± 0.6 33.7± 0.4 31.0± 0.4 BPC 10μg/kg i.p. 14.4± 1.1 † 14.6± 0.3 † 14.8± 0.4 † 14.9± 0.7 † 14.8± 0.4 † 14.7± 0.4 † 14.5± 0.2 † 33.1± 0.8 BPC 10ng/kg i.p. 14.4± 0.3 † 14.2± 0.4 † 14.5± 0.4 † 14.4± 0.7 † 14.2± 0.3 † 14.1± 0.6 † 14.1± 0.4 † QT interval duration (msec) 103.7± 2.2 CONTROL(saline 5ml/kg i.p.) 101.7± 1.8 102± 0.9 101.9± 0.4 101.7± 0.8 101.5± 0.2 101.6± 0.1 101.5± 0.6 101.5± 1.9 BPC 10μg/kg i.p. 68.4± 0.78 † 70.7± 1.5 † 70.1± 1.1 † 70.2± 0.9 † 70.1± 0.4 † 69.8± 1.1 † 68.8± 0.8 † 102.2± 1.7 BPC 10ng/kg i.p. 69.4± 1.0 † 71.5± 1.1 † 70.8± 1.4 † 70.1± 1.3 † 70.2± 0.9 † 71.1± 0.8 † 71.4± 1.2 †

Table 3B: Effects of hypokalemia on amplitudes (0.01mV) of ECG waves, ECG wave duration and interval duration (msec). BPC 157 administered intraperitoneally as a therapy, after furosemide (100 mg/kg intraperitoneally), when hypokalemia- third-degree AV block and ventricular tachycardia were already established. Values are mean ± SEM. †P<0.05 significantly different from control values.