90min after furosemide before intragastric medication Medication at 90 min after furosemide Time after furosemide (post-furosemide time) (min) 95 100 110 120 130 140 150 Time after medication application (post-therapy time) (min) ECG amplitudes of P, R, S, T waves (0.01mV); P wave duration, QRS, QT, RR intervals duration (msec) 5 10 20 30 40 50 60 P wave amplitude (0.01mV) 3.2±0.6 CONTROL(saline 5ml/kg i.g.) 3.1± 0.8 3.3± 0.7 3.2± 0.6 3.6± 0.9 3.5± 1.1 3.5± 1.0 3.5± 0.8 3.1±0.4 BPC 10μg/kg i.g 3.2± 0.3 3.1± 0.4 2.9± 0.8 3.1± 0.7 3.2± 0.6 3.3± 0.7 3.1± 1.0 3.4±0.7 BPC 10ng/kg i.g 3.2± 0.6 3.0± 0.3 3.1± 0.5 3.2± 0.8 3.3± 0.9 3.2± 0.9 3.2± 0.7 R wave amplitude (0.01mV) 32.9±0.6 CONTROL(saline 5ml/kg i.g.) 32.5± 0.9 34.1± 0.3 34.3± 0.6 33.6± 0.4 33.6± 0.8 33.1± 0.3 34.4± 0.9 32.7±0.9 BPC 10μg/kg i.g 36.2± 0.7 42.4± 1.3 † 42.7± 0.8 † 41.9± 1.3 † 43.5± 1.1 † 43.8± 1.7 † 45.1± 1.8 † 33.1±0.8 BPC 10ng/kg i.g 34.2± 0.9 42.1± 0.9 † 42.2± 0.9 † 42.5± 1.1 † 42.8± 0.9 † 43.7± 1.4 † 44.7± 1.3 † S wave amplitude (0.01mV) 15.2±0.2 CONTROL(saline 5ml/kg i.g.)¥ 15.3± 1.1 15.1± 0.5 16.1± 0.4 15.9± 0.6 15.6± 1.1 15.3± 1.2 12.6± 2.6 14.9±0.5 BPC 10μg/kg i.g¥ 15.6± 0.9 21.1± 0.7 † 21.8± 1.2 † 22.2± 1.1 † 23.2± 1.5 † 23.6± 1.7 † 23.1± 1.4 † 15.3±0.8 BPC 10ng/kg i.g¥ 15.9± 0.7 21.1± 0.4 † 21.3± 1.6 † 21.9± 1.0 † 21.4± 0.4 † 21.5± 0.9 † 23.2± 1.6 † T wave amplitude (0.01mV) 1.3±0.4 CONTROL(saline 5ml/kg i.g.)¥ 1.3± 0.1 1.4± 0.8 1.4± 0.1 1.3± 0.9 1.3± 0.9 1.3± 0.1 1.3± 0.2 1.3±0.1 BPC 10μg/kg i.g¥ 2.1± 0.9† 2.3± 0.7 † 2.3± 0.8 † 2.4± 0.1 † 2.4± 0.9 † 2.4± 0.1 † 2.5± 0.1 † 1.4±0.1 BPC 10ng/kg i.g¥ 2.2± 1.3† 2.5± 0.7 † 2.5± 0.3 † 2.4± 0.2 † 2.4± 0.1 † 2.3± 0.4 † 2.3± 0.7 † P wave duration (msec) 22.9±1.2 CONTROL(saline 5ml/kg i.g.) 22.4± 1.2 23.1± 1.3 23.7± 1.3 23.9± 1.8 23.5± 1.4 23.9± 1.2 24.0± 1.1 23.1±1.4 BPC 10μg/kg i.g 22.9± 1.6 18.6± 1.2 † 18.3± 1.1 † 18.5± 1.3 † 18.9± 1.3 † 18.5± 1.4 † 18.8± 1.4 † 23.6±1.6 BPC 10ng/kg i.g 23.1± 1.2 18.9± 1.2 † 19.0± 1.4 † 19.2± 1.5 † 19.0± 1.1 † 19.1± 1.7 † 19.3± 1.3 † QRS interval duration (msec) 31.7±1.4 CONTROL(saline 5ml/kg i.g.) 31.5± 0.8 32.2± 1.4 32.1± 1.6 32.7± 1.4 32.1± 1.2 32.5± 1.6 33.3± 1.4 32.1±1.3 BPC 10μg/kg i.g 31.9± 1.4 18.5± 1.7 † 17.9± 1.2 † 17.7± 1.7 † 17.3± 1.4 † 17.1±1.3 † 17.5± 1.3 † 31.5±1.4 BPC 10ng/kg i.g 31.8± 1.3 19.1± 1.8 † 18.5± 1.4 † 18.2± 1.5 † 18.3± 1.5 † 17.9± 1.6 † 18.1± 1.5 † QT interval duration (msec) 102.9±1.9 CONTROL(saline 5ml/kg i.g.)¥ 102.7± 1.5 102.4± 1.8 102.8± 1.2 102.7± 1.4 102.5± 2.1 101.9± 2.1 102.1± 1.5 102.3±2.1 BPC 10μg/kg i.g¥ 99.2± 1.9 † 76.5± 1.8 † 76.2± 1.6 † 75.9± 1.6 † 75.7± 1.6 † 76.2± 1.8 † 76.3± 1.1 † 102.8±1.8 BPC 10ng/kg i.g¥ 99.4± 1.5 † 77.2± 1.5 † 77.5± 1.7 † 77.3± 1.8 † 76.7± 2.1 † 76.4± 1.7 † 76.1± 1.9 †

Table 4B: Effects of hypokalemia on amplitudes (0.01mV) of ECG waves, ECG wave duration and interval duration (msec). BPC 157 administered intragastrically, as a therapy, after furosemide (100 mg/kg intraperitoneally), when hypokalemia- third-degree AV block and ventricular tachycardia were already established. Values are mean ± SEM. ¥ - Two-way ANOVA with repeated measure on one factor (P<0.05) difference between measurements depends on group membership. †P<0.05 significantly different from control values.