Figure 5: Evaluation by real-time quantitative RT-PCR of the LV mRNA levels of genes related to extracellular matrix remodeling. Results are reported as mean ± SEM (n=10-15/gr). Col I: collagen I; Col III: collagen III; Col IV Fn: fibronectin; LOX1: lysyl oxidase 1; TGFb1: transforming growth factor; MMP2: matrix metallo protease 2 and TIMP-1: tissue inhibitor of metalloprotease 1. Two-way ANOVA statistical analysis results are displayed below each graph. D X T: disease and metformin treatment interaction *: p<0.05 vs. untreated corresponding group from Bonferroni post-test if P<0.05 for D x T.