Figure 3: Effect of perfusion pressure on phosphorylation status of JNK1/2. Hearts were perfused at either 80 or 160 cm H2O and subjected to an ischemia (40 min) reperfusion (15 min) insult followed by freeze clamping for subsequent Western blotting. Densitometry data were initially expressed as the ratio of pJNK1/2 to total JNK1/2 for hearts perfused at each pressure. Thereafter, the pJNK/JNK ratio was expressed as percent of hearts subjected to the low pressure; data are means ± SEM of n=6 hearts/group. Also shown are representative blots for pJNK1/2, JNK1/2 and β-actin for each group. * p<0.05 compared to the other group.