Figure 1: A) Human aortic smooth muscle cells cultured in AngII(1μmol/L) for 48h caused a significantly decreased expression of VSMC contractile marker genes, such as smooth muscle a-actin, smooth muscle 22a, Calponin, but increased expression of VSMC synthetic marker gene such as OPN ,as determined by quantitative reverse-transcription polymerase chain reaction (n=5). *P<0.05 compared with CTRL. B) The expression of contractile marker gene smooth muscle a-actin was decreased , but the expression of OPN was increased, after explored in AngII (1 μmol/L) for 48h, as determined by western blot(n=5), *P<0.05 compared with CTRL.