Figure 4: Pre-treated human aortic smooth muscle cells with inhibitor of autophagy spautin-1 (10 μM) prior to stimulating the cells with Ang II, then the expression of LC3-II,LC3-I,Beclin1 and contractile protein were detected by western blot. Quantification of LC3-II/LC3-I, Beclin1and smooth muscle a-actin were tested (n=5). *P<0.05 compared with untreated cells.