Figure 4: hIL-10 gene delivery and information decoding process in infarcted cardiac tissue. After infarct induction, 50 ml of saline solution containing hIL-10 plasmid was injected retrovenously at 2, 5 or 10 ml/s into the coronary sinus. After 72 hours, pigs were sacrificed and tissue samples were collected from infarcted area for molecular analyses. Quantitative PCR, RT-PCR and ELISA of exogenous hIL-10 gene and transcript in samples of infarcted cardiac tissue were performed, and results were expressed as hIL- 10 DNA, RNA and protein copy number per cell.