Figure 4: Curcumin attenuates doxorubicin cardiotoxicity via regulation of autophagy and apoptosis through JNK phosphorylation. (A) Representative western blot analysis of pJNK, JNK, in post nuclear supernatant of neonatal rat cardiomyocyte. Upper number means loading time of each treatment. Data were normalized with Tubulin which serves as a loading control. Each bar represents mean ± SEM of four independent experiments. *p<0.01, #p<0.05. (B) Representative confocal photomicrograph of cardiomyocyte transfected with GFP-LC3 treated with starvation, DOX, Cur, DOX+Cur over 2 h respectively. Lower tier cells were treated by SP600125 (JNK inhibitor) 1 h prior to each treatment. (C) Representative western blot analysis of LC3 in neonatal rat cardiomyocyte with JNK inhibitor 1 h prior to each treatment over 2 h. β-actin serves as a loading control. Each bar represents mean ± SEM of four independent experiments. *p<0.01. (D) Representative western blot analysis to detect cleaved PARP, pJNK, JNK in post nuclear supernatant of neonatal rat cardiomyocyte with JNK inhibitor over 24 h. Tubulin serves as a loading control. Abbreviations are same as in Fig1. Each bar represents mean ± SEM of four independent experiments. *p<0.01, #p<0.05 NS: Not Significant.