Figure 5: Aging is associated with higher phosphorylation of GSK3β and PTEN in F344XBN rat hearts. Protein isolates of hearts excised from 6-, 27-, 30-, 33- and 36-month rats were analyzed by immunoblotting for changes in total and phosphorylated GSK3β (A) and PTEN (B). Data are mean ±SE (n=6). * indicates significant difference from 6-month group, $ indicates significant difference from 27-month group, # indicates significant difference from 30- month group and @ indicates significant difference from 33-month group (P<0.05).