material |
the form of material |
sample size |
cell dencity
(cells/cm2) |
n |
days in culture |
technical problems |
results /
conclusions |
cell survival |
polylactide |
membrane |
ø 15 mm |
275000-300000 |
11 |
2-12 |
- |
too stiff material |
++ |
chitosan |
membrane |
ø 15 mm |
275000-350000 |
13 |
2-12 |
immunocytochemistry: background |
the cells survived but did not attach as well as on controls |
++ |
chitosan |
gel |
nd |
nd |
nd |
nd |
gelation |
the cells did not spread |
- |
POC |
membrane |
ø 13 mm |
300000-400000 |
2 (POC, POC on CNT), 3 (CNT on POC) |
6-7 |
LIVE/DEAD: background |
the cells survived but there were not as much cells as on controls |
- |
PEOT/PBT |
membrane |
ø 20 mm, thickness 90-130 µm |
335000-414000 |
3 (1000 g/mol), 4 (300 g/mol) |
6 |
- |
the cells survived but there were not as much cells as on controls |
- |
PuraMatrix™ |
gel |
250 µl in 24 well plate |
300000-400000 |
10 (*) |
4-9 |
uneven gelling, the gels detached |
the cells did not migrate inside the gel |
+ |
collagen |
gel from commercial |
250-500 µl in 24 well plate |
331500-350000 |
10 (**) |
nd |
the gels detached |
the cells were not evenly spread |
+ |
collagen |
gel from self isolated |
250 µl in 24 well plate |
400000 (on) / 1400000 (in) |
14 (***) |
4-10 |
- |
the cells were evenly spread and migrated inside the gel |
+++ |
POC = Poly(1,8-octanediol-co-citric acid), CNT = carbon nano tube, PEOT = poly(ethylene oxide terephtalate), PBT = poly(butylene terephtalate)
(*) gel concentration = 0,15-0,4 %; (**) gel concentration = 0,784-1,960 mg/ml; (***) gel concentration = 0,8384 mg/ml |