Table 1

	CFU-Hill	CAC	ECFC
Replating ability	-	-	+
In vitro tube formation	+/-	+/-	+
In vivo de novo vessel formation	-	-	+
Homing to ischemic sites in vivo	+	+	+
Paracrine augmentation of angiogenesis	+	+	+/-
Phagocytosis of bacteria	+	+	-
Clonal proliferative status	-	-	+
Non-specific esterase expression	+	+	-
Phenotypic appearance	CD34+/-	CD34+/-	CD34+
	CD133+	CD133+	CD133-
	VEGFR2+	VEGFR2+	VEGFR2+
	CD45+/-	CD45+/-	CD45-
	CD14+/-	CD14+/-	CD14-
	CD115+	CD115+	CD115-
	CD31+	CD31+	CD31+
	ALDHbright	ALDHbright	ALDHbright/lo
	acLDL uptake	acLDL uptake	acLDL uptake
	UEA-1 lectin binding	UEA-1 lectin binding	UEA-1 lectin binding
	eNos+	eNos+	eNos+
	von Willebrand+	von Willebrand+	von Willebrand+

Properties in bold distinguish cells in ECFC assay from cells in CFU-Hill and CAC assays. The properties credited to an EPC are only fully displayed by ECFC and not CFUHill and CAC. CFU-Hill indicates colony forming unit-Hill; CAC, circulating angiogenic cells; ECFC, endothelial colony forming cells; VEGFR2, vascular endothelial growth factor receptor 2; ALDH, aldehyde dehydrogenase; AcLDL, acetylated low density lipoprotein; UEA-1, Ulex europeaus agglutinin-1; eNOS, endothelial nitric oxide synthase

Table 1: Characteristics and cell surface markers of cells comprising the adherent population in the commonly used assays of “EPC” identification (From Hirschi et al. 2008).