Figure 3: T cells specific for the EDDR1 derived HLA-A2 restricted p15 epitope can be generated from ovarian cancer patients: PBMC from three HLA-A2+ ovarian cancer patients were in vitro stimulated with the p15 synthetic peptide. These cells were tested in an ELISpot assay using T2 cells loaded with the synthetic peptide, normal kidney cell suspensions obtained from a HLA-A2+ healthy donor tissue (with or without exogenously provided p15 peptide) and HLA-A2+ cancer cells lines [breast cancer cell line MDA-MB231 and ovarian cancer cell lines Ovcar3 and SKOV3-A2]. Interferon-γ producing cells were quantitated using Immunospot reader. Error bars represent SEM of experimental replicates.