Figure 5: Immunoflourescent staining for regulatory cells (Tregs) in animals conditioned with total T cells. (A, B, C) Cardiac allograft tissue stained with anti-CD4 (red) and anti-Foxp3 (green) antibody and DAPI, respectively, from animals that had received allochimeric protein (CsA+P) - conditioned total T cells. Arrows denote cluster of CD4+Foxp3+ cell infiltration into the graft. (D, E, F) Cardiac allograft tissue from animals treated with CsA - conditioned total T cells, stained with anti-CD4 (red), anti-Foxp3 (green) antibody and DAPI, respectively. There is no infiltration with CD4+ and Foxp3+ cells. Bar is equal to 50μm.