Donor
|
[[α]1hl/u]-RT1.Aa
|
Low dose CsA
(3 days)
|
High dose CsA
(7 days)
|
Primed
T cells
|
Naïve
T cells
|
Total
T cells
|
CD4+
T cells
|
CD8+
T cells
|
# of Transferred T cells
(secondary recipient)
|
Mean Survival Time |
WF |
- |
- |
- |
+ |
- |
- |
- |
- |
1x107 |
6 days |
WF |
- |
- |
- |
- |
+ |
- |
- |
- |
1x107 |
14.4 days |
WF |
- |
- |
+ |
- |
- |
+ |
- |
- |
1x107 |
>100 days |
WF |
+ |
+ |
- |
- |
- |
+ |
- |
- |
1x107 |
>100 days |
WF |
- |
- |
+ |
- |
- |
- |
+ |
- |
1x107 |
>100 days |
WF |
+ |
+ |
- |
- |
- |
- |
+ |
- |
1x107 |
>100 days |
WF |
- |
- |
+ |
- |
- |
- |
- |
+ |
1x107 |
87 days |
WF |
+ |
+ |
- |
- |
- |
- |
- |
+ |
1x107 |
28 days |
Primary recipients of WF grafts were conditioned with allochimeric class I MHC, [[α]1hl/u]-RT1.Aa (1mg/kg), in addition to 3 days of sub-therapeutic CsA (10mg/kg) or 7
days of high dose CsA alone (10mg/kg). Secondary recipients transplanted with WF grafts then received 1x107 T cells (total, CD4+, or CD8+), peri-operatively, isolated
from a naïve animal or from primary recipients that were either untreated (primed T cells) or conditioned with CsA or CsA+ allochimeric class I MHC (CsA+P). Table shows
expected decreased graft survival in primed and naïve T cells treated groups. There was no difference in graft survival between animals receiving CsA+P - compared to
those that received CsA alone - conditioned cells, nor was there a difference in those that received CD4+ cells. Survival was significantly less in secondary recipients that
had received CD8+ T cells conditioned with CsA+P. |