Figure 3: IFN-γ+ splenocytes elevated significantly after P. pneumotropica infection. (A) Representative FACS plot showing the percentage of IFN-γ+ cells in the different groups as shown in (B). (B–G) Total percentage of IFN-γ+ cells or in different subsets of T cells and NK cells analyzed by FACS. (B) Total percentage of IFN-γ+ cells (C) IFN-γ+CD4+ T cells, (D) IFN-γ+CD8+ T cells, (E) IFN-γ+ NK cells (CD3-NK1.1+), (F) IFN-γ+CD4+ NKT cells (CD3+CD4+NK1.1+ cells), and (G) IFN-γ+CD8+ NKT cells (CD3+CD8+NK1.1+ cells) – for all four groups of mice (non-infected WT and CD1d-/- mice; and infected WT and CD1d-/-). Dot plots show individual values and the mean. One-way ANOVA test with Newman-Keuls multiple comparisons correction was used to evaluate significant differences among the groups; n = 6 mice/ group, *P ≤0.05, **P ≤0.01, ***P ≤0.001.