Figure 4: Elevated IL-17A+ splenocytes particularly in CD8+ T cells while IL-17A+ NKT cells decreased in P. pneumotropica–infected groups. (A) Representative FACS plot showing the percentage of IL-17A+ cells in the different groups as shown in (B). (B–G) Percentage of total IL-17A+ cells and in different subsets of T and NK cells analyzed using FACS. (B) Total percentage of IL-17A+ cells, (C) IL-17A+CD4+ T cells, (D) IL-17A+CD8+ T cells, (E) IL-17A+NK cells (CD3- NK1.1+), (F) IL-17A+CD4+NKT cells, and (G) IL-17A+CD8+ NKT cells – for all four groups of mice (non-infected WT and CD1d-/- mice; and infected WT and CD1d-/-). Dot plots individual values and the mean. One-way ANOVA test with Newman-Keuls multiple comparisons correction was used to evaluate significant differences among the groups; n = 6 mice/group, *P ≤0.05, **P ≤0.01, ***P ≤0.001.