Figure 5: Depletion of NK1.1+ cells using PK136 at an adult age has differential effects on splenocyte populations in infected WT mice. FACS analysis of percentage of (A) CD4+ T cells, (B) CD8+ T cells, (C) the CD4/CD8 ratio in CD3+ T cells, (D) DN cells in CD3+ T cells, (E) NK cells (gated on CD3-NK1.1+DX5+ cells) and (F) CD4+ NKT cells, (G) CD8+ NKT cells, (H) or DN NKT cells for WT mice treated with the NK1.1-depleting antibody PK136 with and without P. pneumotropica infection. Data show mean ± SD. Student’s t-test was used to evaluate significant differences between the groups; n = 3–6 mice/group, **P =0.01, ***P =0.001.