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Figure 1: Gating-strategy of CD4+CD25+CD127-T cells. Strategy for analysis of CD4+CD25+CD127- T cells on fresh drawn blood-samples. % CD25+CD127- were determined in the CD4+CD3+ compartment. CD4+CD25+CD127- cells represent the T regulatory cells. Additional FoxP3 analysis was performed on CD4+CD25+CD127- cells in representative patients. |