Figure 4: In vitro IgM secretion by live CHPV exposed B-cells
The B-cells were exposed to live virus and the exposed cells were incubated up to 96 h post exposure. The culture supernatants were collected at 24 h intervals and tested for CHPV specific IgM using mouse IgM capture ELISA. The mean OD from unexposed B-cell supernatants plus 3 standard deviation (SD) was set as a cut off value.