A) Splenocytes isolated from female PLP-TCR transgenic mice were reprimed
with PLP 139-151 in the presence or absence of 50 µM PTIO. After 4
d, CD4+ T cells were purified and 5 × 106 PLP-specific T cells were transferred
to naïve female SJL/J mice via tail vein. B) Splenocytes isolated from male
PLP-TCR transgenic mice were re-primed with PLP139-151 in the presence
or absence of 100 µM DETA-NONOate. After 4 d, CD4+ T cells were purified
and 5 × 106 PLP-specific T cells were transferred to naïve female SJL/J mice
via tail vein. Six recipient mice (n=6) were used in each group. Mice were
examined for clinical symptoms daily. Data are mean ± SEM of six mice per
group.