A) Splenocytes isolated from female PLP-TCR transgenic mice were reprimed with PLP 139-151 in the presence or absence of 50 M PTIO. After 4 d, CD4+ T cells were purified and 5 106 PLP-specific T cells were transferred to nave female SJL/J mice via tail vein. B) Splenocytes isolated from male PLP-TCR transgenic mice were re-primed with PLP139-151 in the presence or absence of 100 M DETA-NONOate. After 4 d, CD4+ T cells were purified and 5 106 PLP-specific T cells were transferred to nave female SJL/J mice via tail vein. Six recipient mice (n=6) were used in each group. Mice were examined for clinical symptoms daily. Data are mean SEM of six mice per group.
Figure 3: Effect of NO modulators on the encephalitogenicity of PLPspecific T cells.