Splenocytes isolated from MBP-immunized iNOS (-/-) donor mice (B6.129)
were further primed with MBP in the presence or absence of DETA-NONOate
(100 µM). After 4 d, CD4+ T cells were purified and 5 X 106 MBP-primed CD4+ T cells were adoptively transferred to naïve female SJL/J recipient mice.
Similarly, MBP-primed T cells from WT B6.129 mice were also transferred
to naïve female SJL/J mice. T cells from three different donor mice were
transferred to six different recipient mice. Mice were examined for clinical
symptoms daily. Data are mean ± SEM of six mice per group.