 On 14 dpt, spinal cord (A) and cerebellar (B) sections of HBSS-treated (normal),
MBP-primed T cell-treated, PTIO-incubated MBP-primed T cell-treated, and
DNO-incubated MBP primed T cell-treated mice were stained with Luxol fast
blue. Digital images were collected under bright field setting. Demyelination
in spinal cord (C) and cerebellum (D) was represented quantitatively by using
a scale as described in materials and methods. Data are expressed as the
mean ± SEM of five different mice per group. ap < 0.001 vs HBSS (normal); bp
< 0.001 vs MBP-primed T cells; cp < 0.05 vs MBP-primed T cells. Spinal cord
(E) and cerebellum (F) were analyzed for the mRNA expression of CNPase,
MOG and PLP by semi-quantitative RT-PCR. Results represent the analysis
of five different mice per group. |
